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Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients

Received: 5 June 2018     Accepted: 12 July 2018     Published: 8 August 2018
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Abstract

To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P <0.05), but the expression of COX-2 was significantly lower (P <0.05). PPARγ expression in MODS 2 group and MODS 3group was lower than that in vehicle control group, and its expression was progressively decreased during the treatment (P <0.05);in contrast, Expression of COX-2 in MODS2 and MODS3 group was higher than that in vehicle control group, and its level was progressively increased during the treatment (P <0.05). The study revealed a significant negative correlation between COX-2 and PPARγ in MODS1 group, MODS2 group and MODS3 group. (MODS1 group r=-0.761, P<0.01; MODS2 group: r=-0.782, P<0.01; MODS3 group: r=-0.791, P<0.01). There was no significant correlation in vehicle control group (r = -0.185, P >0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.

Published in Clinical Medicine Research (Volume 7, Issue 3)
DOI 10.11648/j.cmr.20180703.14
Page(s) 75-79
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Multiple Organ Dysfunction Syndrome (MODS), Peroxisome Proliferator-Activated (PPARγ), Receptors-γ, Cyclooxygenase-2 (COX-2)

References
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  • APA Style

    Xu Fei, Qiao Wanhai, Wang Liming. (2018). Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clinical Medicine Research, 7(3), 75-79. https://doi.org/10.11648/j.cmr.20180703.14

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    ACS Style

    Xu Fei; Qiao Wanhai; Wang Liming. Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clin. Med. Res. 2018, 7(3), 75-79. doi: 10.11648/j.cmr.20180703.14

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    AMA Style

    Xu Fei, Qiao Wanhai, Wang Liming. Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clin Med Res. 2018;7(3):75-79. doi: 10.11648/j.cmr.20180703.14

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  • @article{10.11648/j.cmr.20180703.14,
      author = {Xu Fei and Qiao Wanhai and Wang Liming},
      title = {Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients},
      journal = {Clinical Medicine Research},
      volume = {7},
      number = {3},
      pages = {75-79},
      doi = {10.11648/j.cmr.20180703.14},
      url = {https://doi.org/10.11648/j.cmr.20180703.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20180703.14},
      abstract = {To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P 0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients
    AU  - Xu Fei
    AU  - Qiao Wanhai
    AU  - Wang Liming
    Y1  - 2018/08/08
    PY  - 2018
    N1  - https://doi.org/10.11648/j.cmr.20180703.14
    DO  - 10.11648/j.cmr.20180703.14
    T2  - Clinical Medicine Research
    JF  - Clinical Medicine Research
    JO  - Clinical Medicine Research
    SP  - 75
    EP  - 79
    PB  - Science Publishing Group
    SN  - 2326-9057
    UR  - https://doi.org/10.11648/j.cmr.20180703.14
    AB  - To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P 0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.
    VL  - 7
    IS  - 3
    ER  - 

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Author Information
  • Department of Emergency Medicine, 3201 Hospital of Xi'an Jiaotong University Health Science Center, Hanzhong, China

  • Department of Emergency Medicine, Second Affiliated Hospital of Xi'an Jiaotong University Health Science Center, Xi’an, China

  • Department of Emergency Medicine, Second Affiliated Hospital of Xi'an Jiaotong University Health Science Center, Xi’an, China

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