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Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients

Received: 4 March 2022     Accepted: 21 March 2022     Published: 14 April 2022
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Abstract

Preeclampsia (PE), which is characterized by hypertension in women who have normal blood pressure before pregnancy and is accompanied by proteinuria, edema, and major organ damage, is the major cause of the increased mortality in pregnant women and perinatal fetuses. So far, genistein is recognized as the only safe and effective natural phytoestrogen without estrogen-like adverse reactions. Recent studies show that genistein may play a significant role in controlling oxidative/nitrative stress during preeclampsia, indicating a possible beneficial role of genistein in the prevention of preeclampsia. However, the ability of genistein to prevent and treat pregnancy-induced hypertension, especially preeclampsia, is unknown. To study the effect of genistein on endothelial cell damage in preeclampsia, we isolated human umbilical vein cells (HUVEC) from 20 normal pregnant women and 40 preeclampsia patients (half treated with genistein and the other half untreated). We found that HUVEC barrier function, proliferation, nitric oxide synthesis, glutathione peroxidase activity, and Bcl-2 expression were significantly decreased in the PE group. Furthermore, 8-hydroxy-2’-deoxyguanosine levels and Bax expression were significantly increased comparing to control group. Genistein treatment reversed these changes in the PE group, suggesting that genistein reduces endothelial cell damage in preeclampsia HUVEC and providing a supporting the use of genistein for PE prevention and treatment.

Published in Clinical Medicine Research (Volume 11, Issue 2)
DOI 10.11648/j.cmr.20221102.13
Page(s) 28-35
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Genistein, Preeclampsia, Human Umbilical Vein Endothelial Cells, Oxidative Stress, Anti-apoptosis

References
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Cite This Article
  • APA Style

    Dan Wu, Lixing Liu, Min Zhang, Xianhui Min. (2022). Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clinical Medicine Research, 11(2), 28-35. https://doi.org/10.11648/j.cmr.20221102.13

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    ACS Style

    Dan Wu; Lixing Liu; Min Zhang; Xianhui Min. Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clin. Med. Res. 2022, 11(2), 28-35. doi: 10.11648/j.cmr.20221102.13

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    AMA Style

    Dan Wu, Lixing Liu, Min Zhang, Xianhui Min. Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clin Med Res. 2022;11(2):28-35. doi: 10.11648/j.cmr.20221102.13

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  • @article{10.11648/j.cmr.20221102.13,
      author = {Dan Wu and Lixing Liu and Min Zhang and Xianhui Min},
      title = {Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients},
      journal = {Clinical Medicine Research},
      volume = {11},
      number = {2},
      pages = {28-35},
      doi = {10.11648/j.cmr.20221102.13},
      url = {https://doi.org/10.11648/j.cmr.20221102.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20221102.13},
      abstract = {Preeclampsia (PE), which is characterized by hypertension in women who have normal blood pressure before pregnancy and is accompanied by proteinuria, edema, and major organ damage, is the major cause of the increased mortality in pregnant women and perinatal fetuses. So far, genistein is recognized as the only safe and effective natural phytoestrogen without estrogen-like adverse reactions. Recent studies show that genistein may play a significant role in controlling oxidative/nitrative stress during preeclampsia, indicating a possible beneficial role of genistein in the prevention of preeclampsia. However, the ability of genistein to prevent and treat pregnancy-induced hypertension, especially preeclampsia, is unknown. To study the effect of genistein on endothelial cell damage in preeclampsia, we isolated human umbilical vein cells (HUVEC) from 20 normal pregnant women and 40 preeclampsia patients (half treated with genistein and the other half untreated). We found that HUVEC barrier function, proliferation, nitric oxide synthesis, glutathione peroxidase activity, and Bcl-2 expression were significantly decreased in the PE group. Furthermore, 8-hydroxy-2’-deoxyguanosine levels and Bax expression were significantly increased comparing to control group. Genistein treatment reversed these changes in the PE group, suggesting that genistein reduces endothelial cell damage in preeclampsia HUVEC and providing a supporting the use of genistein for PE prevention and treatment.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients
    AU  - Dan Wu
    AU  - Lixing Liu
    AU  - Min Zhang
    AU  - Xianhui Min
    Y1  - 2022/04/14
    PY  - 2022
    N1  - https://doi.org/10.11648/j.cmr.20221102.13
    DO  - 10.11648/j.cmr.20221102.13
    T2  - Clinical Medicine Research
    JF  - Clinical Medicine Research
    JO  - Clinical Medicine Research
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    EP  - 35
    PB  - Science Publishing Group
    SN  - 2326-9057
    UR  - https://doi.org/10.11648/j.cmr.20221102.13
    AB  - Preeclampsia (PE), which is characterized by hypertension in women who have normal blood pressure before pregnancy and is accompanied by proteinuria, edema, and major organ damage, is the major cause of the increased mortality in pregnant women and perinatal fetuses. So far, genistein is recognized as the only safe and effective natural phytoestrogen without estrogen-like adverse reactions. Recent studies show that genistein may play a significant role in controlling oxidative/nitrative stress during preeclampsia, indicating a possible beneficial role of genistein in the prevention of preeclampsia. However, the ability of genistein to prevent and treat pregnancy-induced hypertension, especially preeclampsia, is unknown. To study the effect of genistein on endothelial cell damage in preeclampsia, we isolated human umbilical vein cells (HUVEC) from 20 normal pregnant women and 40 preeclampsia patients (half treated with genistein and the other half untreated). We found that HUVEC barrier function, proliferation, nitric oxide synthesis, glutathione peroxidase activity, and Bcl-2 expression were significantly decreased in the PE group. Furthermore, 8-hydroxy-2’-deoxyguanosine levels and Bax expression were significantly increased comparing to control group. Genistein treatment reversed these changes in the PE group, suggesting that genistein reduces endothelial cell damage in preeclampsia HUVEC and providing a supporting the use of genistein for PE prevention and treatment.
    VL  - 11
    IS  - 2
    ER  - 

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Author Information
  • Department of Obstetrics and Gynecology, Putian University, Putian, China

  • Department of Obstetrics and Gynecology, Putian University, Putian, China

  • Department of Obstetrics and Gynecology, Putian University, Putian, China

  • Department of Obstetrics and Gynecology, Clinical Division, 900 Hospital of PLA Department of General Logistics, Fuzhou, China

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